The Resolute® BioSC platform is often a highly modular multi-move chromatography system that may continuously operate a few chromatography separations (in batch or multi-column mode), together with viral inactivation and in-line buffer preparing. The chaining of several unit functions with each other results in a compact and intensified course of action.
The mobile section’s move level is determined by the mixed speeds of the two pumps. By switching the relative speeds of The 2 pumps, diverse binary cellular phases can be prepared.
機械的に高い圧力をかけることによって移動相溶媒を高流速でカラムに通し、これにより分析物が固定相に留まる時間を短くして分離能・検出感度を高くすることを特徴とする。
物質の電気化学的な性質を利用した検出器。pHの変動や酸化還元電位の変動を用いて測定を行う。
Degassing is attained in numerous means, but the commonest are the usage of a vacuum pump or sparging with the inert gas, such as He, that has a very low solubility from the mobile stage. Particulate products, which can clog the HPLC tubing or column, are eradicated by filtering the solvents.
Exactly what is the focus of caffeine in the sample if a 10-μL injection gives a more info peak region of 424195? The data in this issue emanates from Kusch, P.
混合物で構成される試料を分離する。一般にステンレス製の筒の中に、微細な真球状の多孔質シリカゲルをアルキル基等で修飾した物を充填して用いる。分取目的であれば、粉砕シリカゲルも用いられる。
順相クロマトグラフィーは高速液体クロマトグラフィーにおいて最初に使われた。固定相に高極性のもの（シリカゲル）を、移動相に低極性のもの（例えばヘキサン、酢酸エチル、クロロホルムなどの有機溶媒）を用いる。分析物はより極性の高いほどより強く固定相と相互作用して溶出が遅くなる。また極性の高い物質の割合が多い移動相ほど溶出が早くなる。順相タイプは近年の逆相タイプの発展とともに使われることが少なくなったが、順相タイプは逆相タイプをはじめとする他の分離モードとは異なった特性を持つため、目的によっては非常に有効なものとなる。例えば、逆相タイプでは分離が困難なトコフェロールの異性体や保持の困難な糖類を容易に相互分析することができ、また主に水を含まない移動相を用いるので、水に難溶の脂溶性ビタミンや加水分解されやすい酸無水物などの化合物の分離に好適である。
one–one μg of injected analyte. A further limitation of the refractive index detector is the fact that it cannot be utilized for a gradient elution Unless of course the mobile section elements have identical refractive indexes.
System contamination: Dirty HPLC lines, injectors, or detectors can introduce contaminants that show up as ghost peaks. Flush the system with correct solvents to remove any amassed contaminants.
*본 포스팅의 저작권은 써모 피셔 사이언티픽에 있으며, 콘텐츠의 무단 복제 및 수정, 재배포를 금지합니다.
Solvent composition: The ratio of solvents within the cell phase is usually wonderful-tuned to further improve peak resolution and separation.
-hydroxybenzoic acid—on a nonpolar C18 column working with an aqueous buffer of acetic acid and sodium acetate given that the mobile period. The retention times for these weak acids are shorter when utilizing a less acidic cellular period simply because Each and every solute is present within read more an anionic, weak base kind that is less soluble in the nonpolar stationary period.
The liquid that transports the sample with the column is recognized as the mobile stage. It comprises of a number of solvents chosen depending on the Investigation’s distinctive needs.